Publications

Tina L. Tootle. (2025) Prostaglandins limit nuclear actin rod formation during Drosophila oogenesis. microPublication Biology. 10.17912/micropub.biology.001571

Ashley C. Goll*, Kaden H. Bex*, and Tina L. Tootle. (2024). Drosophila melanogaster c306 GAL4 is not specific to ovarian cells. microPublication Biology. 10.17912/micropub.biology.001383.

Nicole M. Green**, Danielle E. Talbot, and Tina L. Tootle**. (Submitted) Nuclear Actin is a Critical Regulator of Drosophila Germline Stem Cell Maintenance. bioRxiv. doi: 10.1101/2024.08.27.609996

**co-corresponding authors

Samuel Q. Mellentine, Hunter N. Brown, Anna S. Ramsey, Jie Li,  and Tina L. Tootle. (2024). Specific prostaglandins are produced in the migratory cells and the surrounding substrate to promote Drosophila border cell migration. Frontiers in Cell and Developmental Biology. Volume 11-2023. Editors’ Showcase 2023: Insights in Cell Adhesion and Migration. doi: 10.3389/fcell.2023.1257751

Michelle S. Giedt*, Jonathon M. Thomalla*, Roger P. White, Matthew R. Johnson, Zon Weng Lai, Tina L. Tootle, and Michael A. Welte. (2023) Adipose triglyceride lipase promotes prostaglandin-dependent actin remodeling by regulating substrate release from lipid droplets. Development. dev.201516. doi: 10.1242/dev.201516

Danielle E. Talbot*, Bailey J. Vormezeele*, Garrett C. Kimble, Dylane M. Wineland, Daniel J. Kelpsch, Michelle S. Giedt and Tina L. Tootle. (2023) Prostaglandins limit nuclear actin to control nucleolar function during oogenesis. Front. Cell Dev. Bio. Volume 11 – 2023. Editors Showcase 2022: Insights in Molecular and Cellular Reproduction.  doi: 10.3389/fcell.2023.1072456

Michelle S. Giedt and Tina L. Tootle. The Vast Utility of Drosophila Oogenesis. Methods Mol Biol. 2626:1-36. doi: 10.1007/978-1-0716-2970-3_1.

In: Giedt, M.S., Tootle, T.L. (eds) Drosophila Oogenesis. Methods in Molecular Biology, vol 2626. Humana, New York, NY.

Lamb MC, Kaluarachchi CP, Lansakara TI, Mellentine SQLan Y, Tivanski AV, and TL. Tootle.  Fascin limits Myosin activity within Drosophila border cells to control substrate stiffness and promote migration. eLIFE. doi.org/10. 7554/eLife. 69836

Green N.M.*, Kimble G.C.*, Talbot D.E.* and T.L. Tootle. (2021) Nuclear Actin. eLS,  1: 958-967. doi:10.1002/9780470015902.a0028471

Palacios V, Kimble G, Tootle TL, and Michael Buszczak. (2021) Importin-9 regulates chromosome segregation and packaging in Drosophila germ cells. JCS. 134(7):jcs258391 doi:10.1101/2020.02.10.942326

Lamb M.C. and T.L. Tootle. (2020) Fascin in Cell Migration: More Than an Actin Bundling Protein. Biology.  9:403. doi:10.3390/biology9110403

Fox E.F., Lamb M.C*., Mellentine S.Q*. and T.L. Tootle. (2020) Prostaglandins regulate invasive, collective border cell migration. MBoC. 15: 1584-1594. doi:10.1091/mbc.E19-10-0578

Lamb M.C., Anliker K.K., and T.L. Tootle. (2020) Fascin regulates protrusions and delamination to mediate invasive, collective cell migration in vivo. Developmental Dynamics. 249: 961-982. doi:10.1002/dvdy.186

FitzSimons M., Beauchemin , Kelpsch D., Lamb M., Tootle, T.L., and Yin, V. (2020) Cardiac injury modulates critical components of prostaglandin E2 signaling during zebrafish heart regeneration. Scientific Reports. 10: 3095. doi:10.1038/s41598-020-59868-6

Duan T., Green N.M., Tootle, T.L., and P Geyer. (2020). Nuclear architecture in Drosophila female GSCs. Current Opinion in Insect Science. Invited Review. 37: 30-38. doi: 10.1016/j.cois.2019.11.007

Tootle T.L., Hoffmann D., Allen A., Spracklen A., Groen C., and D Kelpsch. (2019) Mini-course based undergraduate research experience: Impact on student understanding of STEM research and interest in STEM programs. Journal of College Science Teaching. 48:44-54

Wineland D*, Kelpsch D*, Tootle TL. (2018). Multiple pools of nuclear actin. The Anatomical Record. 301(12):2014-2036.

Kelpsch D, Tootle TL. (2018). Nuclear actin: from discovery to function. The Anatomical Record. 301(12):1999-2013

Article about the lab:

Understanding development and disease: prostaglandins coordinate actin cytoskeletal remodeling.  Research Features 110: 26-29.

Jayo A, Malboubi M, Antoku S, Chang W, Ortiz-Zapater E, Groen C, Pfisterer K, Tootle T, Charras G, Gundersen G.G., Parsons M. 2016. Fascin Regulates Nuclear Movement and Deformation in Migrating Cells. Dev. Cell. 38:371-383.

Kelpsch DJ, Groen CM*, Fagan TN*, Sudhir S, Tootle TL. 2016. Fascin regulates nuclear actin during Drosophila oogenesis. MBoC. 27:2965-297.

Spracklen AJ, Tootle TL. 2015. Drosophila – a model for studying prostaglandin signaling. Bioactive Lipid Mediators: Current Reviews and Protocols. Springer Japan. Editors: Takehiko Yokomizo and Makoto Murakami.

Groen CM, Tootle TL. 2015. Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers. Drosophila oogenesis: Methods and Protocols. Springer Methods Mol Biol. Editors: Diana Bratu and Gerard McNeil. 1328:113-24.

Groen CM, Jayo A, Parsons M, Tootle TL. (2015) Prostaglandins regulate Fascin localization to the nucleus and function in nucleolar architecture. MBoC. 328:113-24.

Spracklen AJ, Fagan TN, Lovander KE, Tootle TL. (2014)  The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis. Dev. Biol. 393(2):209-26.

Spracklen AJ, Kelpsch DJ, Chen X, Spracklen CN, Tootle TL. (2014) Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis. MBoC. 25(3):397-411.

Spracklen AJ, Tootle TL. (2013) The utility of stage-specific mid-to-late Drosophila follicle isolation. J Vis Exp. 2013 Dec 2;(82). doi: 10.3791/50493.

Tootle TL. (2013) Genetic insights into the in vivo functions of prostaglandin signaling. Int J Biochem Cell Biol. 45(8):1629-32.

Groen CM*, Spracklen AJ*, Fagan TN, Tootle TL (2012) Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling. MBoC. 23(23)4567-4578.

Tootle TL, Williams D, Hubb A, Frederick R, Spradling A (2011) Drosophila eggshell production: identification of new genes and coordination by Pxt. PLoS One 6: e19943.

Tootle TL, Spradling AC (2008) Drosophila Pxt: a cyclooxygenase-like facilitator of follicle maturation. Development 135: 839-47